Pharmacological Screening of Centella asiatica, Glycerrhiza glabra, Gymnosporia montana and Cow Urine for Controlling Side Effects of Neuroleptics as well as their Use in Various Neurological Disorders

The main objective of the present investigation is to explore polyherbal containing Centella asiatica, Glycerrhiza glabra, Gymnosporia montana and Cow urine for its neuroleptic properties, using the tail suspension test (TST), light dark test (LDM) and elevated plus maze (EPM) test on mice. Pharmacognostic and pharmacological study of aerial part of Centella asiatica, root of Glycerrhiza glabra and Leaves of Gymnosporia montana was carried out. Polyherbal was found safe up to a dose of 5000 mg/kg since no mortality was observed within 48 hrs. of administration. In TST, it has shown a dose-dependent decrease in immobility time, which is an indication of its antidepressant effect, in LDM it had significantly exhibited anxiolytic activity by increasing time spent and number of crossings in light compartment. decreased the time spent in dark compartment and decreased the number of rearings in both light and dark compartments. Similarly in EPM model had significantly enhanced both number of entries and time spent in open arms and decreased in number of entries and time spent in closed arms. Phytochemical investigation revealed the presence of saponins, tannins, glycosides, carbohydrates, phenolic compounds, flavonoids. Alcoholic extracts of polyherbal were found to produce significant (P<0.05) neuroleptic action. In all the model, the extract at 1000mg/kg showed significant activity. Thus the result recorded with above experimental models confirms the neuroleptic action of used polyherbal extract along with cow urine.